Composite

Part:BBa_K300008:Design

Designed by: Lorenzo Pasotti, Matteo Meroso, Nicolo' Politi   Group: iGEM10_UNIPV-Pavia   (2010-10-05)

Cm resistance and R6K replication origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used BioBrick Standard Assembly.


Source

BBa_P1004 - 2009 DNA Distribution;

BBa_B0015 - 2009 DNA Distribution;

BBa_J61001 - 2010 DNA Distribution.

References

[1] Kleanthous C, Shaw W (1984), Analysis of the mechanism of chloramphenicol acetyltransferase by steady-state kinetics, Biochem. J. 223, 211-220.

[2] Haldimann A, Wanner BL (2001), Conditional-replication, integration, excision, and retrieval plasmid-host systems for gene structure-function studies of bacteria. Journal of Bacteriology, 183(21), p.6386-6393.